Employing the concept of gender as a situated performance, my research explores negotiations of gender performances by bisexuals in interpersonal relationships and how such performances may alter due to the sex/gender of their partner. Previous studies of gender performance have primarily focused on heterosexual women and men. Also, the existing research on bisexuality has largely concentrated on the gender of bisexuals’ relationship partners, particularly the perceived differences between women and men. By focusing on the gender performances of bisexuals themselves, this research is a contribution to the budding academic literature on bisexuality as well as the sociological study of gender. I examine how bisexuals utilize traditional scripts of femininity and masculinity in their relationships with women and men. Also, due to the dichotomization of heterosexuality and homosexuality, male/female sexes, and the corresponding genders of masculinity and femininity, bisexuals must negotiate an alteration of traditional performances of gender and sexuality.